File:Altered-responsiveness-to-extracellular-ATP-enhances-acetaminophen-hepatotoxicity-1478-811X-11-10-S9.ogv
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Altered-responsiveness-to-extracellular-ATP-enhances-acetaminophen-hepatotoxicity-1478-811X-11-10-S9.ogv (Ogg Theora video file, length 21 s, 240 × 240 pixels, 240 kbps, file size: 603 KB)
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[edit]DescriptionAltered-responsiveness-to-extracellular-ATP-enhances-acetaminophen-hepatotoxicity-1478-811X-11-10-S9.ogv |
English: Additional file 9: Video 7 Detection of intracellular Ca2+ signals with confocal microscopy – APAP-treated primary hepatocytes displayed sustained calcium signal following ATP administration. Nuclear and cytosolic Ca2+ were monitored in individual cells by using time-lapse confocal microscopy. Primary mouse hepatocytes were cultured on glass coverslips in a density of 3×105 cells/well in 6 wells plates and kept in a Hepes-buffered solution during experiments. Cells were incubated with APAP (20 mM) and after 6 hours 4 μM cell permeant Fluo4-AM (fluo-4 acetoxymethyl ester; Molecular Probes) was added to the culture. ATP (10 μm) was used to trigger intracellular calcium signal. |
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Source | Amaral S, Oliveira A, Marques P, Quintao J, Pires D, Resende R, Sousa B, Melgaco J, Pinto M, Russo R, Gomes A, Andrade L, Zanin R, Pereira R, Bonorino C, Soriani F, Lima C, Cara D, Teixeira M, Leite M, Menezes G (2013). "Altered responsiveness to extracellular ATP enhances acetaminophen hepatotoxicity". Cell Communication and Signaling. DOI:10.1186/1478-811X-11-10. PMID 23384127. PMC: 3608937. | ||
Author | Amaral S, Oliveira A, Marques P, Quintao J, Pires D, Resende R, Sousa B, Melgaco J, Pinto M, Russo R, Gomes A, Andrade L, Zanin R, Pereira R, Bonorino C, Soriani F, Lima C, Cara D, Teixeira M, Leite M, Menezes G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 06:20, 7 April 2013 | 21 s, 240 × 240 (603 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 9: Video 7 |
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Author | Amaral S, Oliveira A, Marques P, Quintao J, Pires D, Resende R, Sousa B, Melgaco J, Pinto M, Russo R, Gomes A, Andrade L, Zanin R, Pereira R, Bonorino C, Soriani F, Lima C, Cara D, Teixeira M, Leite M, Menezes G |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |