File:Afferent-Neurons-of-the-Zebrafish-Lateral-Line-Are-Strict-Selectors-of-Hair-Cell-Orientation-pone.0004477.s004.ogv
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Afferent-Neurons-of-the-Zebrafish-Lateral-Line-Are-Strict-Selectors-of-Hair-Cell-Orientation-pone.0004477.s004.ogv (Ogg Theora video file, length 29 s, 344 × 178 pixels, 83 kbps, file size: 293 KB)
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[edit]DescriptionAfferent-Neurons-of-the-Zebrafish-Lateral-Line-Are-Strict-Selectors-of-Hair-Cell-Orientation-pone.0004477.s004.ogv |
English: This time-lapse sequence shows the dynamics of re-innervation of a neuromast during regeneration (Figure 7). A single mem-tdTomato-labeled neuron is followed in a SqET4 transgenic fish. This sequence clearly shows the initial trial-and-error exploratory behavior of the neuron is refined over time. The dendritic arbor re-localizes to establish synaptic contacts exclusively with all the hair cells on the same side of the line of mirror symmetry. The movie starts 20 hours after a 45 min exposure of the animal to 250 µM of neomycin. The larva was mounted in 1% low-melting-point agarose, maintained at 21°C and images were acquired with a Leica TCS SPE confocal microscope with a 40× oil immersion objective every 5 minutes during 17 hours. Z-stacks were acquired at 0.8 µm intervals. |
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Source | Movie S3 from Faucherre A, Pujol-Martí J, Kawakami K, López-Schier H (2009). "Afferent Neurons of the Zebrafish Lateral Line Are Strict Selectors of Hair-Cell Orientation". PLOS ONE. DOI:10.1371/journal.pone.0004477. PMID 19223970. PMC: 2637426. | ||
Author | Faucherre A, Pujol-Martí J, Kawakami K, López-Schier H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:28, 30 October 2012 | 29 s, 344 × 178 (293 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |