File:Active-Sulforhodamine-101-Uptake-into-Hippocampal-Astrocytes-pone.0049398.s001.ogv
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Active-Sulforhodamine-101-Uptake-into-Hippocampal-Astrocytes-pone.0049398.s001.ogv (Ogg Theora video file, length 10 s, 770 × 504 pixels, 132 kbps, file size: 166 KB)
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[edit]DescriptionActive-Sulforhodamine-101-Uptake-into-Hippocampal-Astrocytes-pone.0049398.s001.ogv |
English: This movie is a two-photon z-stack recording (70 µm, 2 µm, 0.5 min−1) of a SR101-staining experiment with 3 different SR101-concentrations. After recording one z-stack without SR101, 0.1 µM SR101 was bath-applied, 20 minutes later the SR101-concentration was increased to 1 µM for 20 minutes. Finally, 10 µM SR101 was applied for 20 minutes before the recording was stopped. The white spots identify EGFP-labeled astrocytes that were selected for analysis of fluorescence intensities (Figure 5). The blue spot marks the position where fluorescence background within the slice was recorded, while the purple spot was located above the slice to record fluorescence changes of the bath solution (Scale bar 40 µm). |
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Source | Movie S1 from Schnell C, Hagos Y, Hülsmann S (2012). "Active Sulforhodamine 101 Uptake into Hippocampal Astrocytes". PLOS ONE. DOI:10.1371/journal.pone.0049398. PMID 23189143. PMC: 3506634. | ||
Author | Schnell C, Hagos Y, Hülsmann S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:15, 3 December 2012 | 10 s, 770 × 504 (166 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |