File:Activation-of-the-NLRP3-Inflammasome-by-IAV-Virulence-Protein-PB1-F2-Contributes-to-Severe-ppat.1003392.s007.ogv
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Size of this JPG preview of this OGG file: 672 × 599 pixels. Other resolutions: 269 × 240 pixels | 538 × 480 pixels | 861 × 768 pixels | 1,146 × 1,022 pixels.
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[edit]DescriptionActivation-of-the-NLRP3-Inflammasome-by-IAV-Virulence-Protein-PB1-F2-Contributes-to-Severe-ppat.1003392.s007.ogv |
English: Inhibition of phagocytosis blocks PB1-F2-induced ASC speck formation. NLRP3-deficient macrophages reconstituted with ASC-cerulean (blue) and NLRP3 were pre-cultured in optical culture dishes (Ibidi) and treated with 20 µM of Latrunculin A for 40 mins prior to stimulation with pHrodo-labeled (red) PB1-F2 peptide. Cells were visualized in 3D by z-stack collection (6 confocal planes) every 6 mins for 90 mins. Movie is a maximum intensity projection of 3D image series from Imaris. Movie is representative of two independent experiments. |
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Source | Video S3 from McAuley J, Tate M, MacKenzie-Kludas C, Pinar A, Zeng W, Stutz A, Latz E, Brown L, Mansell A (2013). "Activation of the NLRP3 Inflammasome by IAV Virulence Protein PB1-F2 Contributes to Severe Pathophysiology and Disease". PLOS Pathogens. DOI:10.1371/journal.ppat.1003392. PMID 23737748. PMC: 3667782. | ||
Author | McAuley J, Tate M, MacKenzie-Kludas C, Pinar A, Zeng W, Stutz A, Latz E, Brown L, Mansell A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:03, 13 June 2013 | 1.4 s, 1,146 × 1,022 (443 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | McAuley J, Tate M, MacKenzie-Kludas C, Pinar A, Zeng W, Stutz A, Latz E, Brown L, Mansell A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-05 |