File:Activation-of-the-NLRP3-Inflammasome-by-IAV-Virulence-Protein-PB1-F2-Contributes-to-Severe-ppat.1003392.s005.ogv

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Original file (Ogg Theora video file, length 22 s, 1,602 × 880 pixels, 383 kbps, file size: 1.03 MB)

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English: PR8 PB1-F2 peptide induces ASC speck formation. NLRP3-deficient macrophages reconstituted with ASC-cerulean (blue) and NLRP3 were cultured in optical culture dishes (Ibidi) and treated with pHrodo-labeled (red) PB1-F2 peptide and visualized in 3D by z-stack collection (6 confocal planes) every 6 mins for 3.5 hours. Rapid phagocytosis of PB1-F2 peptide induces increased fluorescence of labeled peptide due to decreased pH of phagolysosomal compartment. Uptake of PB1-F2 peptide induces oligomerization of ASC in cytosol to form multimeric ASC speck. Movie is a maximum intensity projection of 3D image series from Imaris. Movie is representative of three independent experiments.
Date
Source Video S1 from McAuley J, Tate M, MacKenzie-Kludas C, Pinar A, Zeng W, Stutz A, Latz E, Brown L, Mansell A (2013). "Activation of the NLRP3 Inflammasome by IAV Virulence Protein PB1-F2 Contributes to Severe Pathophysiology and Disease". PLOS Pathogens. DOI:10.1371/journal.ppat.1003392. PMID 23737748. PMC: 3667782.
Author McAuley J, Tate M, MacKenzie-Kludas C, Pinar A, Zeng W, Stutz A, Latz E, Brown L, Mansell A
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Date/TimeThumbnailDimensionsUserComment
current02:02, 13 June 201322 s, 1,602 × 880 (1.03 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 177 kbps Completed 17:46, 16 August 2018 7.0 s
VP9 480P 92 kbps Completed 17:46, 16 August 2018 5.0 s
VP9 360P 49 kbps Completed 17:45, 16 August 2018 4.0 s
VP9 240P 27 kbps Completed 17:45, 16 August 2018 4.0 s
WebM 360P 27 kbps Completed 02:03, 13 June 2013 2.0 s
QuickTime 144p (MJPEG) 61 kbps Completed 01:50, 15 October 2024 1.0 s

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