File:Accumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s012.ogv
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Accumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s012.ogv (Ogg Theora video file, length 22 s, 180 × 180 pixels, 659 kbps, file size: 1.73 MB)
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[edit]DescriptionAccumulative-Difference-Image-Protocol-for-Particle-Tracking-in-Fluorescence-Microscopy-Tested-in-pone.0012216.s012.ogv |
English: The movies report the reconstruction of the trajectory of one SLN in the cells (target 11 in Fig. 4). The images are reported in direct gray levels (all the other movies are in inverted gray levels). The trajectory lasts for 440 frames and therefore two different color rendering is given here. In this movie one every two frames are inserted in the movie and the whole trajectory is rendered in jet(128) colormap on all the frames of the movie. It is noteworthy that, despite the high density of targets in the image and the several interactions between them (20<frames<110, 180<frame<210; 320<frame<440) the trajectory is fully reconstructed with a low level of ambiguity. |
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Source | Movie S10 from Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G (2010). "Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes". PLOS ONE. DOI:10.1371/journal.pone.0012216. PMID 20808918. PMC: 2923183. | ||
Author | Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:40, 28 May 2013 | 22 s, 180 × 180 (1.73 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Villa C, Caccia M, Sironi L, D'Alfonso L, Collini M, Rivolta I, Miserocchi G, Gorletta T, Zanoni I, Granucci F, Chirico G |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The movies report the reconstruction of the trajectory of one SLN in the cells (target 11 in Fig. 4). The images are reported in direct gray levels (all the other movies are in inverted gray levels). The trajectory lasts for 440 frames and therefore two different color rendering is given here. In this movie one every two frames are inserted in the movie and the whole trajectory is rendered in jet(128) colormap on all the frames of the movie. It is noteworthy that, despite the high density of targets in the image and the several interactions between them (20<frames<110, 180<frame<210; 320<frame<440) the trajectory is fully reconstructed with a low level of ambiguity. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |