File:A-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s3.ogv
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A-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s3.ogv (Ogg Theora video file, length 6.6 s, 130 × 130 pixels, 138 kbps, file size: 111 KB)
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[edit]DescriptionA-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s3.ogv |
English: Supplementary Movie 2 Monitoring of LacNAc-treated TIL conjugates under flow stress conditions. LacNAc-treated CD8 TILs from patient LB3450 (Supplementary Table 1) were stained with CFSE (green) and allowed to interact for 30 min with CMTMR (red)-loaded sAg-pulsed B cells. A flow of pre-warmed medium was generated by progressively increasing the pressure up to 2 bar, by steps of 0.1 bar. The movie starts at 0.5 bar. TIL detachment from their targets was monitored by video microscopy. Scale bar represents 10 μm. Frames were collected at 4-second intervals. Display rate: 15 frames/second. |
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Source | Video file from Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P (2016). "A major secretory defect of tumour-infiltrating T lymphocytes due to galectin impairing LFA-1-mediated synapse completion". Nature Communications. DOI:10.1038/ncomms12242. PMID 27447355. PMC: 4961845. | ||
Author | Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:56, 26 October 2016 | 6.6 s, 130 × 130 (111 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 2 |
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Author | Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Monitoring of LacNAc-treated TIL conjugates under flow stress conditions. LacNAc-treated CD8 TILs from patient LB3450 (Supplementary Table 1) were stained with CFSE (green) and allowed to interact for 30 min with CMTMR (red)-loaded sAg-pulsed B cells. A flow of pre-warmed medium was generated by progressively increasing the pressure up to 2 bar, by steps of 0.1 bar. The movie starts at 0.5 bar. TIL detachment from their targets was monitored by video microscopy. Scale bar represents 10 μm. Frames were collected at 4-second intervals. Display rate: 15 frames/second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-07-22 |