File:A-gene-trap-transposon-eliminates-haematopoietic-expression-of-zebrafish-Gfi1aa-but-does-not-mmc2.ogv

English: Movie 1 : In the gene trap line qmc551, GFP is expressed in primitive red blood cells and in spindle-shaped cells located between the dorsal aorta and the posterior cardinal vein at 26 hpf. This movie is related to Figure 1. (A) Live movie of the trunk and tail of a 26 hpf qmc551 transgenic. GFP is expressed in circulating and stationary blood cells and in elongated cells located between the dorsal aorta and the posterior cardinal vein. Anterior is to the left, dorsal is up. Pictures of this timelapse movie were taken every 100 ms with a Hamamatsu Orca-ER camera on a Nikon SMZ1500 dissection microscope with an epifluorescence attachment using a FITC filter set. The camera was controlled by IP lab software. Images were pseudo-colored in IP lab and saved as TIFF files. The series of TIFF files was imported into Imaris and turned into a video that was then annotated in iMovie. (B) Confocal analysis of the trunk of a fixed 26 hpf qmc551 transgenic embryo shows expression of GFP in blood cells and in spindle-shaped cells located between the lumen of the dorsal aorta and the lumen of the vein. An animation of a 3D maximum intensity projection of a 67.5 µm thick Z stack is shown. A still image of the maximum intensity projection of the Z stack highlights GFP+endothelial cells (green arrows), red blood cells in circulation (red arrow) and outside the vessels (red arrowheads). The embryo was first treated with an overdose of anesthetic and then fixed in 4% PFA for an hour. The Z-stack was taken on an inverted Zeiss Exciter confocal microscope and has the dimensions 161×161×67.5 µm, respectively. The movie starts with anterior to the left and dorsal up. The confocal images were acquired on a Zeiss Exciter microscope with an EC Plan-Neofluar 40x/1.30 Oil DIC M27 lens.