File:A-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s008.ogv
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[edit]DescriptionA-Role-for-the-Chaperone-Complex-BAG3-HSPB8-in-Actin-Dynamics-Spindle-Orientation-and-Proper-pgen.1005582.s008.ogv |
English: Related to Fig 3B —BAG3 depletion impairs mitotic spindle dynamics. Video microscopy of a representative siBAG3-treated HeLa cells expressing RFP-a-tubulin and GFP-actin, which show characteristic defects in mitotic spindle dynamics upon depletion of BAG3 (siBAG3). Mitotic cells were imaged for a 1 h-period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective; single plane images are displayed at 7 frames/sec. |
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Source | S2 Movie from Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J (2015). "A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis". PLOS Genetics. DOI:10.1371/journal.pgen.1005582. PMID 26496431. PMC: 4619738. | ||
Author | Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J | ||
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current | 12:45, 8 December 2015 | 8.3 s, 680 × 680 (768 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Related to Fig 3B —BAG3 depletion impairs mitotic spindle dynamics. |
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Author | Fuchs M, Luthold C, Guilbert S, Varlet A, Lambert H, Jetté A, Elowe S, Landry J, Lavoie J |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Video microscopy of a representative siBAG3-treated HeLa cells expressing RFP-a-tubulin and GFP-actin, which show characteristic defects in mitotic spindle dynamics upon depletion of BAG3 (siBAG3). Mitotic cells were imaged for a 1 h-period at ~1.5 min intervals using a Perkin Elmer UltraVIEW Spinning Disc Confocal and 40x air 0.75NA objective; single plane images are displayed at 7 frames/sec. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-10-23 |