File:A-Quantitative-Comparison-of-Human-HT-1080-Fibrosarcoma-Cells-and-Primary-Human-Dermal-Fibroblasts-pone.0081689.s029.ogv

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A-Quantitative-Comparison-of-Human-HT-1080-Fibrosarcoma-Cells-and-Primary-Human-Dermal-Fibroblasts-pone.0081689.s029.ogv ‎(Ogg Theora video file, length 5.3 s, 243 × 347 pixels, 590 kbps, file size: 382 KB)

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English: HT-1080s split into multiple migrating cells or cell fragments in collagen. Time-lapse images (15 min / frame) illustrating a migrating HT-1080 in collagen (1.7 mg/mL) that splits into two motile fragments without mitotic rounding (also Figure S10H). The formation of multiple motile fragments by HT-1080s during migration resembled IMMP formation (see Yount et. al., J. Neuro-Oncol. 2007) such as demonstrated in synthetic ECM (Movie S17). However, IMMP formation was not confirmed in collagen, and therefore the observations shown here could be due to other abnormal cell division phenomena. Time shown in Hr:Min. Scale bar = 50 μm.
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Source Movie S18 from Schwartz M, Rogers R, Singh S, Lee J, Loveland S, Koepsel J, Witze E, Montanez-Sauri S, Sung K, Tokuda E, Sharma Y, Everhart L, Nguyen E, Zaman M, Beebe D, Ahn N, Murphy W, Anseth K (2013). "A Quantitative Comparison of Human HT-1080 Fibrosarcoma Cells and Primary Human Dermal Fibroblasts Identifies a 3D Migration Mechanism with Properties Unique to the Transformed Phenotype". PLOS ONE. DOI:10.1371/journal.pone.0081689. PMID 24349113. PMC: 3857815.
Author Schwartz M, Rogers R, Singh S, Lee J, Loveland S, Koepsel J, Witze E, Montanez-Sauri S, Sung K, Tokuda E, Sharma Y, Everhart L, Nguyen E, Zaman M, Beebe D, Ahn N, Murphy W, Anseth K
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