File:A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv005.ogv
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A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv005.ogv (Ogg Theora video file, length 7.7 s, 360 × 120 pixels, 431 kbps, file size: 403 KB)
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[edit]DescriptionA-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv005.ogv |
English: Movie of ARF1 Activity with PI-3K Activity Blocked by LY294002 RAW264.7 macrophages co-transfected with ARF1-CFP and YFP-NGAT demonstrated high FRET signals in the area of their Golgi network in the presence of LY294002: the nucleus is visible in the phase contrast image shown at the left; next to the nucleus, the crescent-shaped region of high E D (far-right panel) corresponds to the Golgi apparatus. The Golgi is also visible in the R M image (center panel) where ARF1-CFP is present in excess over YFP-NGAT. Cells treated with LY294002 could bind opsonized particles and form phagocytic cups but could not complete phagocytosis. At the upper left and right of the macrophage, binding of opsonized erythrocytes was not accompanied by the activation of ARF1-CFP. This result indicates that amplification of FcγR-signaling by PI-3K is required for the activation of ARF1 at the phagosome. |
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Source | Video S5 from Beemiller P, Hoppe A, Swanson J (2006). "A Phosphatidylinositol-3-Kinase-Dependent Signal Transition Regulates ARF1 and ARF6 during Fcγ Receptor-Mediated Phagocytosis". PLOS Biology. DOI:10.1371/journal.pbio.0040162. PMID 16669702. PMC: 1457017. | ||
Author | Beemiller P, Hoppe A, Swanson J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:06, 16 November 2012 | 7.7 s, 360 × 120 (403 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Movie of ARF1 Activity with PI-3K Activity Blocked by LY294002 |
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Author | Beemiller P, Hoppe A, Swanson J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | RAW264.7 macrophages co-transfected with ARF1-CFP and YFP-NGAT demonstrated high FRET signals in the area of their Golgi network in the presence of LY294002: the nucleus is visible in the phase contrast image shown at the left; next to the nucleus, the crescent-shaped region of high E D (far-right panel) corresponds to the Golgi apparatus. The Golgi is also visible in the R M image (center panel) where ARF1-CFP is present in excess over YFP-NGAT. Cells treated with LY294002 could bind opsonized particles and form phagocytic cups but could not complete phagocytosis. At the upper left and right of the macrophage, binding of opsonized erythrocytes was not accompanied by the activation of ARF1-CFP. This result indicates that amplification of Fc?R-signaling by PI-3K is required for the activation of ARF1 at the phagosome. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2006-06 |
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June 2006
application/ogg
ec90a627de1b8c247c95f7d8da54ea51454b2485
412,571 byte
7.66666666666667 second
120 pixel
360 pixel
Categories:
- Videos of cell biology
- In vitro
- ADP-ribosylation factor 1
- ADP-ribosylation factors
- Brefeldin A
- Videos of cultured cells
- Enzyme activation
- Fluorescence resonance energy transfer
- Videos of macrophages
- Phagocytosis
- Phosphatidylinositol 3-kinases
- IgG receptors
- Recombinant fusion proteins
- Videos of signal transduction