File:A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv003.ogv
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A-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv003.ogv (Ogg Theora video file, length 8.3 s, 360 × 120 pixels, 402 kbps, file size: 409 KB)
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[edit]DescriptionA-Phosphatidylinositol-3-Kinase-Dependent-Signal-Transition-Regulates-ARF1-and-ARF6-during-Fcγ-pbio.0040162.sv003.ogv |
English: Movie of ARF1 Activation during Phagocytosis by a BFA-Treated Macrophage Panels in the video, left to right: phase contrast, R M and E D images from a macrophage expressing ARF1-CFP and YFP-NGAT, and treated with 5 μM BFA for 30 min prior to microscopic observation. Exposure to BFA prevented the activation of ARF1 at the Golgi—no regions of high FRET are visible near the nucleus in the E D image at the start of the movie. However, the two phagocytic events (lower right, followed by upper left) led to the activation of ARF1, indicating that ARF1 activation at the phagosome is mediated by a BFA-resistant ARNO/cytohesin-family GEF. |
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Source | Video S3 from Beemiller P, Hoppe A, Swanson J (2006). "A Phosphatidylinositol-3-Kinase-Dependent Signal Transition Regulates ARF1 and ARF6 during Fcγ Receptor-Mediated Phagocytosis". PLOS Biology. DOI:10.1371/journal.pbio.0040162. PMID 16669702. PMC: 1457017. | ||
Author | Beemiller P, Hoppe A, Swanson J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:06, 16 November 2012 | 8.3 s, 360 × 120 (409 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Movie of ARF1 Activation during Phagocytosis by a BFA-Treated Macrophage |
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Author | Beemiller P, Hoppe A, Swanson J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Panels in the video, left to right: phase contrast, R M and E D images from a macrophage expressing ARF1-CFP and YFP-NGAT, and treated with 5 ?M BFA for 30 min prior to microscopic observation. Exposure to BFA prevented the activation of ARF1 at the Golgi?no regions of high FRET are visible near the nucleus in the E D image at the start of the movie. However, the two phagocytic events (lower right, followed by upper left) led to the activation of ARF1, indicating that ARF1 activation at the phagosome is mediated by a BFA-resistant ARNO/cytohesin-family GEF. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2006-06 |
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June 2006
application/ogg
e6582d24dd2d0ce7c142949e1c2f997b00d04d8a
418,312 byte
8.33333333333333 second
120 pixel
360 pixel
Categories:
- Videos of cell biology
- In vitro
- ADP-ribosylation factor 1
- ADP-ribosylation factors
- Brefeldin A
- Videos of cultured cells
- Enzyme activation
- Fluorescence resonance energy transfer
- Videos of macrophages
- Phagocytosis
- Phosphatidylinositol 3-kinases
- IgG receptors
- Recombinant fusion proteins
- Videos of signal transduction