File:A-Novel-Cervical-Spinal-Cord-Window-Preparation-Allows-for-Two-Photon-Imaging-of-T-Cell-video 4.ogv
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[edit]DescriptionA-Novel-Cervical-Spinal-Cord-Window-Preparation-Allows-for-Two-Photon-Imaging-of-T-Cell-video 4.ogv |
English: Intraluminal crawling of an activated 2D2 CD4+ T cell in inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). In vitro activated 2D2 CD4+ T-cells labeled with fluorescent CellTracker CMAC were systemically injected via the carotid artery catheter into a surgically prepared mouse at the onset of EAE. In the regions of interest, one transferred CD4+ T cell was observed to crawl along the direction of blood flow until 4 min and 20 s of recording. At this time, the T cell changed the direction of crawling against the blood flow until minute 12 of the recording. At this time point, the observed T cell again changed the direction of crawling along the direction of blood flow and continued to crawl to the end of 20 min of recording. A x–y–t time-lapse sequence of a 200 μm × 200 μm scan field at a depth of 79–100 μm and 8 z-stacks with 3 µm spacing is shown. Blood vessels were labeled by injection of Alexa Fluor 594 conjugated anti-endoglin antibody. CMAC (blue, CD4+ T cell) and anti-endoglin (red, blood vessels) were excited at 780 nm. Time is shown in minutes and seconds. Scale bar: 20 μm. |
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Source | Video S4 from Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B (2017). "A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of T-Cell Interactions with the Cervical Spinal Cord Microvasculature during Experimental Autoimmune Encephalomyelitis". Frontiers in Immunology. DOI:10.3389/fimmu.2017.00406. PMID 28443093. PMC: 5387098. | ||
Author | Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 15:19, 20 May 2017 | 8.7 s, 1,046 × 1,046 (2.62 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Intraluminal crawling of an activated 2D2 CD4+ T cell in inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). In vitro activated 2D2 CD4+ T-cells labeled with fluorescent CellTracker CMAC were systemically injected via the carotid artery catheter into a surgically prepared mouse at the onset of EAE. In the regions of interest, one transferred CD4+ T cell was observed to crawl along the direction of blood flow until 4 min and 20 s of recording. At this time, the T cell changed the direction of crawling against the blood flow until minute 12 of the recording. At this time point, the observed T cell again changed the direction of crawling along the direction of blood flow and continued to crawl to the end of 20 min of recording. A x–y–t time-lapse sequence of a 200 μm × 200 μm scan field at a depth of 79–100 μm and 8 z-stacks with 3 µm spacing is shown. Blood vessels were labeled by injection of Alexa Fluor 594 conjugated anti-endoglin antibody. CMAC (blue, CD4+ T cell) and anti-endoglin (red, blood vessels) were excited at 780 nm. Time is shown in minutes and seconds. Scale bar: 20 μm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-04-11 |