File:A-Novel-Cervical-Spinal-Cord-Window-Preparation-Allows-for-Two-Photon-Imaging-of-T-Cell-video 2.ogv
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[edit]DescriptionA-Novel-Cervical-Spinal-Cord-Window-Preparation-Allows-for-Two-Photon-Imaging-of-T-Cell-video 2.ogv |
English: Arrest of activated 2D2 GFP CD4+ T cells within inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). Active EAE was induced as described in Section “Methods.” Laminectomy was performed (described in step 15 of the procedures) on day 22 post-immunization when the mouse showed onset of disease. In vitro activated CD4+ T cells from “2D2-GFP”-mice were injected via a carotid catheter before 2P-IVM imaging. Blood vessels were labeled by injection of Alexa Fluor 594 conjugated anti-endoglin antibody. GFP (green, CD4+ T cells) and anti-endoglin (red, blood vessels) were excited at 780 nm. A x–y–t time-lapse sequence of a 400 μm × 400 μm scan field at a depth of 47–91 μm and 12 z-stacks with 4 µm spacing shows the arrest of CD4+ T cells within post-capillary venules of the cervical spinal cord. Firmly arrested T cells are seen to neither move nor detach from the vessel wall within a timeframe of 20 s. Time is shown in minutes and seconds. Scale bar: 50 μm. |
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Source | Video S2 from Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B (2017). "A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of T-Cell Interactions with the Cervical Spinal Cord Microvasculature during Experimental Autoimmune Encephalomyelitis". Frontiers in Immunology. DOI:10.3389/fimmu.2017.00406. PMID 28443093. PMC: 5387098. | ||
Author | Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 15:18, 20 May 2017 | 4.0 s, 697 × 697 (1.57 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Haghayegh Jahromi N, Tardent H, Enzmann G, Deutsch U, Kawakami N, Bittner S, Vestweber D, Zipp F, Stein J, Engelhardt B |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Arrest of activated 2D2 GFP CD4+ T cells within inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). Active EAE was induced as described in Section “Methods.” Laminectomy was performed (described in step 15 of the procedures) on day 22 post-immunization when the mouse showed onset of disease. In vitro activated CD4+ T cells from “2D2-GFP”-mice were injected via a carotid catheter before 2P-IVM imaging. Blood vessels were labeled by injection of Alexa Fluor 594 conjugated anti-endoglin antibody. GFP (green, CD4+ T cells) and anti-endoglin (red, blood vessels) were excited at 780 nm. A x–y–t time-lapse sequence of a 400 μm × 400 μm scan field at a depth of 47–91 μm and 12 z-stacks with 4 µm spacing shows the arrest of CD4+ T cells within post-capillary venules of the cervical spinal cord. Firmly arrested T cells are seen to neither move nor detach from the vessel wall within a timeframe of 20 s. Time is shown in minutes and seconds. Scale bar: 50 μm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2017-04-11 |