File:A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv
A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv (Ogg Theora video file, length 6.0 s, 512 × 512 pixels, 3.54 Mbps, file size: 2.53 MB)
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[edit]DescriptionA-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv |
English: Morphological changes and movement of cells in the presence of MVs. Time-lapse observation of a monolayer of Ca9.22 cells was monitored by an optical microscope (Olympus) equipped with a CCD camera (Andor) in the presence of highly purified MVs isolated by the SB technique. The protein concentration of MVs used in the assay was 20 µg/ml. Images were taken at 30 sec/frame for 30 min immediately after addition of MVs suspended in Eagle’s balanced salt solution (EBSS). The constructed time-lapse images are shown at 100 msec/frame (300 times the original speed). The width of the image is 170 µm. |
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Source | Video S1 from Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H (2014). "A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner". PLOS ONE. DOI:10.1371/journal.pone.0095137. PMID 24830438. PMC: 4022494. | ||
Author | Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:03, 22 May 2014 | 6.0 s, 512 × 512 (2.53 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |