File:A-Capsid-Encoded-PPxY-Motif-Facilitates-Adenovirus-Entry-ppat.1000808.s008.ogv

English: Comparison of intracellular dynamics for VI-wt vs. VI-M1 vs. VI-wt (+ nocodazole). U2OS cells were transfected with VI-wt (left and right panel) or VI-M1 (middle panel) fused to mRFP. The cell to the right was treated with nocodazole (5 µg/ml) prior to image acquisition. Movies were acquired using a Nikon TE 2000 microscope equipped with Cascade 512B 2 camera at 1 frame per second. The movies were labeled and assembled using imageJ and converted into QuickTime™ movies. All three displayed movies were taking at the same frame rate. The movies show rapid intracellular movement of VI-wt at nearly real-time (left). VI-M1 shows strongly reduced movement (middle). Treatment of VI-wt transfected cells with nocodazole, strongly reduces the VI-wt movement resembling VI-M1 dynamics (right).