File:A-C.-trachomatis-Cloning-Vector-and-the-Generation-of-C.-trachomatis-Strains-Expressing-Fluorescent-pone.0057090.s010.ogv

English: Time-lapse video microscopy of C. trachomatis inclusion fusion 24 h post infection. Video S2 shows the fusion of inclusions harboring GFP or mCherry expressing C. trachomatis. HeLa cells were co-infected with a C. trachomatis strain that expressed GFP (green) or mCherry (red) under the control of the incD promoter. 24 h post infection, a cell containing 2 GFP- and 1 mCherry-positive inclusion was monitored by spinning disc confocal microscopy. A z-stack of 56 images covering 14 µm was acquired every 20 minutes. The QuickTime video was generated using Improvision Volocity. The time (hours: minutes:seconds) is indicated in the upper right corner. The video is played at 2 frames/s. Scale Bar: 8 µm.