File:3D-Quantitative-Imaging-of-Unprocessed-Live-Tissue-Reveals-Epithelial-Defense-against-Bacterial-pone.0024008.s009.ogv
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3D-Quantitative-Imaging-of-Unprocessed-Live-Tissue-Reveals-Epithelial-Defense-against-Bacterial-pone.0024008.s009.ogv (Ogg Theora video file, length 12 s, 512 × 492 pixels, 441 kbps, file size: 658 KB)
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[edit]Description3D-Quantitative-Imaging-of-Unprocessed-Live-Tissue-Reveals-Epithelial-Defense-against-Bacterial-pone.0024008.s009.ogv |
English: The reflection method was used to enable visualization of both live and dead cells (red), while the autofluorescence method showed only live cells (detects cytoplasmic NAD(P)H of actively metabolizing cells) (magenta). The two channels were superimposed (fuchsia) to confirm that penetrated bacteria (green) in tissue-paper blotted MyD88 (−/−) mouse corneas were actually overlaid with cells, and that some overlying cells remained viable. Note the large number of bacteria at the interface between the epithelium and the stroma (appears red) at the level of the basal lamina barrier. |
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Source | Video S9 from Tam C, LeDue J, Mun J, Herzmark P, Robey E, Evans D, Fleiszig S (2011). "3D Quantitative Imaging of Unprocessed Live Tissue Reveals Epithelial Defense against Bacterial Adhesion and Subsequent Traversal Requires MyD88". PLOS ONE. DOI:10.1371/journal.pone.0024008. PMID 21901151. PMC: 3162028. | ||
Author | Tam C, LeDue J, Mun J, Herzmark P, Robey E, Evans D, Fleiszig S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:03, 29 May 2013 | 12 s, 512 × 492 (658 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Tam C, LeDue J, Mun J, Herzmark P, Robey E, Evans D, Fleiszig S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The reflection method was used to enable visualization of both live and dead cells (red), while the autofluorescence method showed only live cells (detects cytoplasmic NAD(P)H of actively metabolizing cells) (magenta). The two channels were superimposed (fuchsia) to confirm that penetrated bacteria (green) in tissue-paper blotted MyD88 (?/?) mouse corneas were actually overlaid with cells, and that some overlying cells remained viable. Note the large number of bacteria at the interface between the epithelium and the stroma (appears red) at the level of the basal lamina barrier. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
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2011
application/ogg
e8283ce52c5e845dadf761e1d78a385904c01399
674,162 byte
12.24 second
492 pixel
512 pixel
Categories:
- Innate immunity
- Host-pathogen interactions
- Videos of epithelium
- Videos of Pseudomonas infections
- Infectious disease modeling
- Bacterial infections of the eye and adnexa
- Bacterial adhesion
- Inbred CBA mice
- Knockout mice
- Myeloid differentiation factor 88
- Videos of Pseudomonas aeruginosa
- Videos of signal transduction
- Toll-like receptors
- Media from PLOS ONE