File:3-D-neurohistology-of-transparent-tongue-in-health-and-injury-with-optical-clearing-Movie2.ogv

English: In-depth visualization and projection of tongue innervation at the mucosal boundary and muscle domain. Specimen was labeled with nuclear dye(green) and pan-neuronal marker PGP9.5 (white). First part: gross view. Second part: zoom-in examination of muscle domain. The continuous display of the PGP9.5-labeled nerves and 360° projection of the image stack show the tongue innervation with a 3-D space continuum and without loss of signals at the center of the image stack (which otherwise would indicate limitation of antibody penetration in immunohistochemistry). Imaging depth: 200 μm.