File:2-Bromopalmitate-Reduces-Protein-Deacylation-by-Inhibition-of-Acyl-Protein-Thioesterase-Enzymatic-pone.0075232.s001.ogv
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[edit]Description2-Bromopalmitate-Reduces-Protein-Deacylation-by-Inhibition-of-Acyl-Protein-Thioesterase-Enzymatic-pone.0075232.s001.ogv |
English: Deacylation of N13GAP-43(C3S) at different doses of 2-BP. CHO-K1 cells expressing N13GAP-43(C3S)-YFP (pseudocolored green) were incubated in DMEM supplemented with CHX and protein degradation inhibitors at 20°C on the microscope stage, following the experimental procedure described in Fig. 2A. Cells were treated with 25, 50 or 150 µM 2-BP or DMSO (Control), and time series were acquired (1 frame/min) for the following 20 min, as indicated in Materials and methods. |
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Source | Movie S1 from Pedro M, Vilcaes A, Tomatis V, Oliveira R, Gomez G, Daniotti J (2013). "2-Bromopalmitate Reduces Protein Deacylation by Inhibition of Acyl-Protein Thioesterase Enzymatic Activities". PLOS ONE. DOI:10.1371/journal.pone.0075232. PMC: 3788759. | ||
Author | Pedro M, Vilcaes A, Tomatis V, Oliveira R, Gomez G, Daniotti J | ||
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current | 13:52, 7 October 2013 | 6.0 s, 4,096 × 1,512 (7.65 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Pedro M, Vilcaes A, Tomatis V, Oliveira R, Gomez G, Daniotti J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Date and time of digitizing | 2013 |
Language | English |