File:“cAMP-Sponge”-A-Buffer-for-Cyclic-Adenosine-3′-5′-Monophosphate-pone.0007649.s006.ogv
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“cAMP-Sponge”-A-Buffer-for-Cyclic-Adenosine-3′-5′-Monophosphate-pone.0007649.s006.ogv (Ogg Theora video file, length 7.4 s, 196 × 137 pixels, 791 kbps, file size: 717 KB)
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[edit]Description“cAMP-Sponge”-A-Buffer-for-Cyclic-Adenosine-3′-5′-Monophosphate-pone.0007649.s006.ogv |
English: Vectorial transfer of cAMP between connected cells. NCM460 cells stably expressing the EpacH30 cAMP sensor were transiently transfected with cAMP sponge. Here the microscope field contained an isolated control cell (on the left) and a couplet of cells in which only one cell expressed the sponge construct (on the right). The image sequence shows a significant delay in the PGE2 response of the coupled sponge-expressing cell compared to the two controls, with an apparent transfer of cAMP from the control (top cell in couplet) to the cell expressing the cAMP sponge (bottom). Time-lapse images represent pseudo-color of EpacH30 480 nm/535 nm FRET emission ratio, one frame every 10 seconds, total experiment duration 5.5 minutes. |
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Source | Movie S1 from Lefkimmiatis K, Moyer M, Curci S, Hofer A (2009). "“cAMP Sponge”: A Buffer for Cyclic Adenosine 3′, 5′-Monophosphate". PLOS ONE. DOI:10.1371/journal.pone.0007649. PMID 19888343. PMC: 2766031. | ||
Author | Lefkimmiatis K, Moyer M, Curci S, Hofer A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:13, 17 February 2017 | 7.4 s, 196 × 137 (717 KB) | Revent (talk | contribs) | fix, with correct bitrate | |
07:25, 17 February 2017 | 7.4 s, 196 × 137 (246 KB) | Revent (talk | contribs) | Reverted to version as of 18:02, 18 November 2012 (UTC) | ||
22:45, 15 February 2017 | 7.4 s, 196 × 137 (209 KB) | Revent (talk | contribs) | fix | ||
18:02, 18 November 2012 | 7.4 s, 196 × 137 (246 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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