File:Β1-Integrin-Maintains-Integrity-of-the-Embryonic-Neocortical-Stem-Cell-Niche-pbio.1000176.s009.ogv

English: Time lapse imaging of organotypic brain slice prepared 24 h after in utero electroporation with eGFP-F DNA into a wild-type E14.5 embryo and incubated in the presence of a drop of β1 integrin blocking antibody-containing growth factor–reduced matrigel in the lateral ventricle. Automated multipoint scanning using a multiphoton laser (850 nm) was used to monitor the behavior of the eGFP-F+ cells. In presence of β1 blocking antibody, the neuroepithelial organization of the VZ appears to be progressively disrupted because of an alteration of the radial morphology of the NSC cells whose both basal and apical processes appear bowed and exhibit detachment of their ventricular end feet. Scale bar represents 25 µm.