File:Β-Globin-LCR-and-Intron-Elements-Cooperate-and-Direct-Spatial-Reorganization-for-Gene-Therapy-pgen.1000051.s006.ogv
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[edit]DescriptionΒ-Globin-LCR-and-Intron-Elements-Cooperate-and-Direct-Spatial-Reorganization-for-Gene-Therapy-pgen.1000051.s006.ogv |
English: Example 3D DNA FISH reconstruction of transgene localization in uninduced MEL BGT158 cells. The transgene is detected as a red signal by a 10 kb DIG labeled probe using Alexa Fluor 546 labeled tyramide signal amplification with DAPI counterstained nuclear DNA in green. DAPI-rich areas correspond to heterochromatin. |
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Source | Video S1 from Buzina A, Lo M, Moffett A, Hotta A, Fussner E, Bharadwaj R, Pasceri P, Garcia-Martinez J, Bazett-Jones D, Ellis J (2008). "β-Globin LCR and Intron Elements Cooperate and Direct Spatial Reorganization for Gene Therapy". PLOS Genetics. DOI:10.1371/journal.pgen.1000051. PMID 18404216. PMC: 2271131. | ||
Author | Buzina A, Lo M, Moffett A, Hotta A, Fussner E, Bharadwaj R, Pasceri P, Garcia-Martinez J, Bazett-Jones D, Ellis J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:40, 17 November 2012 | 12 s, 1,372 × 954 (1.27 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Buzina A, Lo M, Moffett A, Hotta A, Fussner E, Bharadwaj R, Pasceri P, Garcia-Martinez J, Bazett-Jones D, Ellis J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Example 3D DNA FISH reconstruction of transgene localization in uninduced MEL BGT158 cells. The transgene is detected as a red signal by a 10 kb DIG labeled probe using Alexa Fluor 546 labeled tyramide signal amplification with DAPI counterstained nuclear DNA in green. DAPI-rich areas correspond to heterochromatin. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-04 |
Categories:
- Gene expression
- Videos of gene therapy
- Sickle cell anemia
- Base sequence
- Binding sites
- Cell lines
- DNA primers
- Genetic enhancer elements
- Genetic complementation test
- Genetic vectors
- Globins
- Locus control region
- Transgenic mice
- Octamer transcription factor-1
- Recombinant proteins
- Reverse transcriptase polymerase chain reaction