Category:Media from Justine & Poddubnaya - 10.1051/parasite/2018007
(2018). "Spermiogenesis and spermatozoon ultrastructure in basal polyopisthocotylean monogeneans, Hexabothriidae and Chimaericolidae, and their significance for the phylogeny of the Monogenea". Parasite 25: 7. DOI:10.1051/parasite/2018007. ISSN 1776-1042.
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Abstract
Sperm ultrastructure provides morphological characters useful for understanding phylogeny; no study was available for two basal branches of the Polyopisthocotylea, the Chimaericolidea and Diclybothriidea. We describe here spermiogenesis and sperm in Chimaericola leptogaster (Chimaericolidae) and Rajonchocotyle emarginata (Hexabothriidae), and sperm in Callorhynchocotyle callorhynchi (Hexabothriidae). Spermiogenesis in C. leptogaster and R. emarginata shows the usual pattern of most Polyopisthocotylea with typical zones of differentiation and proximo-distal fusion of the flagella. In all three species, the structure of the spermatozoon is biflagellate, with two incorporated trepaxonematan 9 + “1” axonemes and a posterior nucleus. However, unexpected structures were also seen. An alleged synapomorphy of the Polyopisthocotylea is the presence of a continuous row of longitudinal microtubules in the nuclear region. The sperm of C. leptogaster has a posterior part with a single axoneme, and the part with the nucleus is devoid of the continuous row of microtubules. The spermatozoon of R. emarginata has an anterior region with membrane ornamentation, and posterior lateral microtubules are absent. The spermatozoon of C. callorhynchi has transverse sections with only dorsal and ventral microtubules, and its posterior part shows flat sections containing a single axoneme and the nucleus. These findings have important implications for phylogeny and for the definition of synapomorphies in the Neodermata. We point out a series of discrepancies between actual data and interpretation of character states in the matrix of a phylogeny of the Monogenea. Our main conclusion is that the synapomorphy “lateral microtubules in the principal region of the spermatozoon” does not define the Polyopisthocotylea but is restricted to the Mazocraeidea.
Abbreviations for all figures
A-CM: Anterior extremity of cortical microtubules
A-IM: Anterior extremity of internal microtubules
AM: Arching membrane
A-Mit: Anterior extremity of mitochondrion
A-N: Anterior extremity of nucleus
ASE: Anterior spermatozoon extremity
Ax, Ax1, Ax2: Axonemes
AZ: Attachment zone
C, C1, C2: Centrioles
CM: Cortical microtubules
D: Dense zone
FF: Free flagellum
IB: Intercentriolar body
IM: Internal microtubules
M: (in micrographs), Mit (in diagrams) Mitochondrion
MCE: Median cytoplasmic process
MT: microtubules
N: Nucleus
O: Ornamentation on membrane
P-Ax1, P-Ax2: Posterior extremity of axoneme 1, 2
PSE: Posterior spermatozoon extremity
P-CM: Posterior extremity of cortical microtubules
P-IM: Posterior extremity of internal microtubules
P-Mit: Posterior extremity of mitochondrion
P-N: Posterior extremity of nucleus
SR: Striated root
Media in category "Media from Justine & Poddubnaya - 10.1051/parasite/2018007"
The following 17 files are in this category, out of 17 total.
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Parasite170144-fig01 spermiogenesis Chimaericola leptogaster.png 2,399 × 2,968; 6.39 MB
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Parasite170144-fig02 spermiogenesis Chimaericola leptogaster.png 1,871 × 1,886; 3.72 MB
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Parasite170144-fig03 spermatozoon Chimaericola leptogaster.png 2,404 × 2,814; 8.95 MB
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Parasite170144-fig04 spermatozoon Chimaericola leptogaster.png 2,327 × 1,477; 4.68 MB
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Parasite170144-fig05 spermiogenesis Rajonchocotyle emarginata.png 2,262 × 1,343; 4.58 MB
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Parasite170144-fig06 spermiogenesis Rajonchocotyle emarginata.png 2,335 × 2,724; 9.15 MB
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Parasite170144-fig07 spermiogenesis Rajonchocotyle emarginata.png 2,319 × 2,371; 7.84 MB
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Parasite170144-fig08 spermiogenesis Rajonchocotyle emarginata.png 2,322 × 1,828; 6.36 MB
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Parasite170144-fig09 spermatozoon Rajonchocotyle emarginata.png 2,333 × 3,305; 9.55 MB
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Parasite170144-fig10 spermatozoon Rajonchocotyle emarginata.png 2,405 × 3,193; 8.7 MB
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Parasite170144-fig11 spermatozoon Callorhynchocotyle callorhynchi.png 2,315 × 3,029; 9.52 MB
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Parasite170144-fig12 spermatozoon Callorhynchocotyle callorhynchi.png 2,332 × 2,649; 7.49 MB
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Parasite170144-fig13 spermatozoon Chimaericola leptogaster (drawing).png 2,638 × 3,518; 741 KB
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Parasite170144-fig14 spermatozoon Rajonchocotyle emarginata (drawing).png 3,556 × 3,538; 1.08 MB
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Parasite170144-fig15 spermatozoon Callorhynchocotyle callorhynchi (drawing).png 3,543 × 3,547; 976 KB
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Parasite170144-fig17 Phylogenetic trees of Polyopisthocotylea.png 8,188 × 3,783; 827 KB