Category:Media from Igeh et al.- 10.1051/parasite/2017046

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(2017). "Redescription of Cichlidogyrus philander (Monogenea, Ancyrocephalidae) using scanning electron microscopy (SEM) and molecular analysis". Parasite 24: 49. DOI:10.1051/parasite/2017046. ISSN 1776-1042.

Redescription of Cichlidogyrus philander (Monogenea, Ancyrocephalidae) using scanning electron microscopy (SEM) and molecular analysis. 

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Instance of	scholarly article
Main subject	Cichlidogyrus Ancyrocephalidae (Ancyrocephalidae)
Author	Quinton M Dos Santos (2) Annemarie Avenant-Oldewage (3) Patience C Igeh (1)
Published in	Parasite
Publication date	1 December 2017
Authority file  Q46250303 DOI: 10.1051/PARASITE/2017046 PubMed publication ID: 29194034 PMC publication ID: 5711379 Reasonator Scholia Wikidocumentaries PetScan statistics WikiMap Locator tool KML file Search depicted

Abstract
The sclerotized structures of monogeneans have traditionally been studied by light microscopy and different staining techniques. Recently, enzymatic digestion followed by scanning electron microscopy (SEM) has enabled the examination of structural details not visible with light microscopy. In order to obtain better, and more accurate, morphological information on sclerotized structures not affected by mounting medium or cover slip pressure, the sclerites of Cichlidogyrus philander Douëllou, 1993 (Monogenea, Ancyrocephalidae), collected from Pseudocrenilabrus philander (Weber, 1897), were redescribed using SEM. Parasites were collected from Padda Dam, Gauteng, South Africa and soft tissue was digested to release the sclerotized structures. The digested tissue also provided sufficient genetic material for molecular characterization of this species. Cichlidogyrus philander is characterised by a penis with a sharp, curved, lateral termination, an accessory piece with a hook-like extremity that may appear forked terminally, and lack of a visible vagina. The transverse bars have concave and convex surfaces with ribs on the concave surface. The dorsal bar bears fenestrations at the base of the auricles and the ventral and dorsal gripi are dissimilar. Furthermore, the large first pair of uncinuli shows lateral wings on the left side of the base. On top of this wing, a ball-like structure with a small fenestration is visible. Genetic characters derived from the 28S rDNA, the COI mitochondrial DNA and ITS1 rDNA regions distinguish C. philander from all other Cichlidogyrus sequenced species.