Category:José María Requena

**José María Requena**
	Spanish biochemist, ORCID id # 0000-0001-7550-0198
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Date of birth	27 May 1962; Tragacete
Educated at	Autonomous University of Madrid;
Occupation	senior lecturer; university teacher;
Employer	Autonomous University of Madrid;
Field of work	Leishmania infantum JPCM5; Leishmania braziliensis MHOM/BR/75/M2904; Leishmania donovani HU3; Leishmania major Friedlin;
Sibling	Teresa Requena;
Notable work	A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi; Complete nucleotide sequence of the hsp70 gene of T. cruzi; Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes; The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes of Drosophila hydei.; Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA.; A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei; DNA binding properties and antileukemic (L1210) activity of a Pt-pentamidine complex; The mRNA coding for the nucleosomal protein H2A ofLeishmaniais polyadenylated and has stem-loops at the 3′ end; Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification; Molecular characterization of a Leishmania donovani infantum antigen identified as histone H2A; Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase; Bacteriophage lambda: long-term stored stocks ready for lysis; Mapping of antigenic determinants of the T. cruzi hsp70 in chagasic and healthy individuals; Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells; Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania.; Isolation of Trypanosoma cruzi specific nuclear repeated DNA sequences; Cytoplasmic-nuclear translocation of the Hsp70 protein during environmental stress in Trypanosoma cruzi; The anti Z-DNA reactivity of Z-DNA forming sequences is affected by platinum antitumor drugs.; The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain; During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins; Assignment of genes to Leishmania infantum chromosomes: karyotype and ploidy; Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi genome project; Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis; Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis; During canine viscero-cutaneous leishmaniasis the anti-Hsp70 antibodies are specifically elicited by the parasite protein; Genomic repetitive DNA elements of Trypanosoma cruzi; Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.; Organization, transcription and regulation of the Leishmania infantum histone H3 genes; Mapping of the linear antigenic determinants of the Leishmania infantum hsp70 recognized by leishmaniasis sera; Presence of antibodies in the aqueous humour and cerebrospinal fluid during Leishmania infections in dogs. Pathological features at the central nervous system; …;
Authority file
	Q58252822; VIAF ID: 260412672; IdRef ID: 170542807; ORCID iD: 0000-0001-7550-0198; ResearcherID: I-2022-2019
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