File:Zebrafish Structural requirements for Syndapin operation.jpg
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| Description |
Figure 7. Structural requirements for Syndapin operation. (A) Schematic depiction of the various Syndapin mutants analyzed. (B) Quantitative phenotypic analysis of normal (blue) or abnormal (black) pacsin3 (3 ng MO) morphants coinjected with 25 pg Syndapin WT (n = 185) or Syndapin 1–305 (n = 97), 1–423 (n = 98) or 5KRE (n = 121) mutant mRNAs. Bar = 250 mm. (C–F) Representative images of the phenotype of 24 hpf pacsin3 MO embryos expressing the indicated Syndapin proteins. (G) Immunoblot analysis of expression of various Syndapin fragments and mutants from transfected pCS2+ in HeLa cells. The anti-Syndapin serum recognizes the EFC domain; as positive controls, purified Syndapin (1–304) and a Drosophila head lysate containing endogenous Syndapin were used. (H–I) Representative images of pacsin3 MO embryos also expressing D. rerio Pacsin2 or Pacsin3 SH3D proteins. (J) Quantitative phenotypic analysis of normal (blue) or abnormal (black) pacsin3 (3 ng MO) morphants alone (n = 50) or coinjected with 25 pg Syndapin WT (n = 185), LMKKRGAAA (n = 119), LMKRGAA (n = 99), LMRGA (n = 95; 50 pg, n = 48; 100 pg, n = 43), KKRAA (n = 122; 50 pg, n = 57; 100 pg, n = 76) or 25 pg of both the LMRGA and KKRAA (n = 115) mutant Syndapin mRNAs. (K–P) Representative images of the phenotype of pacsin3 MO embryos expressing the indicated Syndapin proteins |
| Date | |
| Source | Edeling MA, Sanker S, Shima T, Umasankar PK, Ho¨ ning S, et al. (2009) Structural Requirements for PACSIN/Syndapin Operation during Zebrafish Embryonic Notochord Development. PLoS ONE 4(12): e8150. doi:10.1371/journal.pone.0008150 https://d-scholarship.pitt.edu/13299/1/Structural_Requirements.pdf doi:10.1371/journal.pone.0008150.g007 |
| Author | James Keen, Thomas Jefferson University, United States of America |
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© 2009 Edeling et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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| current | 20:52, 27 April 2024 | | 1,091 × 717 (525 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 7. Structural requirements for Syndapin operation. (A) Schematic depiction of the various Syndapin mutants analyzed. (B) Quantitative phenotypic analysis of normal (blue) or abnormal (black) pacsin3 (3 ng MO) morphants coinjected with 25 pg Syndapin WT (n = 185) or Syndapin 1–305 (n = 97), 1–423 (n = 98) or 5KRE (n = 121) mutant mRNAs. Bar = 250 mm. (C–F) Representative images of the phenotype of 24 hpf pacsin3 MO embryos expressing the indicated Syndapin pro... |
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