File:Thermal Proteome Profiling.png
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Captions
Captions
An example thermal proteome profiling workflow.
Summary[edit]
| Description |
English: A cellular lysate is split into control (vehicle) and treatment (compound) groups. Each group is split into aliquots, and each aliquot is heated to a different temperature. As the sample temperature increases, proteins denature and precipitate out of solution. When a test compound is added, target proteins are protected from denaturation by ligand-induced stabilization. After heating, soluble proteins in the supernatant are quantified. Quantification of proteins allows for the generation of protein melting curves. When melting curves shift to the right, a protein is thought to be stabilized by the treatment compound. |
| Date | |
| Source | BioRender.com |
| Author | BioRender.com |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:31, 6 December 2021 | | 4,058 × 4,972 (1.02 MB) | Sgold626 (talk | contribs) | Uploaded a work by BioRender.com from BioRender.com with UploadWizard |
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