File:The-Digital-Fish-Library-Using-MRI-to-Digitize-Database-and-Document-the-Morphological-Diversity-of-pone.0034499.g005.jpg

English: (A) This damba, Paretroplus damii (AMNH 248081, SL 76 mm) was imaged at 7T with the following 3D FLASH pulse sequence parameters: FA = 15°, TR = 24.07 ms, TE = 11.92 ms, slice thickness = 100 µm, slice matrix = 300×1024×500, resolution = 100 µm3, averages = 2. MRI slice data is overlaid with a selection of segmented soft tissue structures color-coded as follows: light blue = brain and spinal cord; dark blue = eyes; magenta = vestibular labyrinth; green = gas bladder; light brown = alimentary tract; red = heart and dorsal aorta. (B) Both MRI and CT data were acquired for this island kelpfish, Alloclinus holderi (SIO 67-272, SL 94 mm). A selection of soft tissue structures were segmented from MRI data and are color-coded as follows: blue = eyes; green = brain; red = alimentary tract; light brown = ovaries; purple = liver. Segmented soft tissues were co-registered with a volumetric rendering of CT imaged hard tissue structures acquired by The University of Texas High-Resolution X-ray Computed Tomography Facility (UTCT) (slice thickness 42 µm). (C) This smooth hammerhead, Sphyrna zygaena (SIO 64-528; SL 104 mm) was imaged at 3T with the following 3D FSPGR pulse sequence parameters: FA = 35°, TR = 9.86 ms, TE = 4.11 ms, slice thickness = 900 µm, slice matrix = 512×512×236, resolution = 683 µm3, averages = 2. It was scanned in 4 sections and pieced together in Amira for segmentation. A selection of segmented soft tissue structures are color-coded as follows: brown = alimentary tract; dark blue = eyes; green = olfactory system; light blue = brain; magenta = dorsal aorta; puce = liver; red = heart; white = spinal cord.