File:Schematic depiction of two affinity purification approaches..jpg

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Schematic_depiction_of_two_affinity_purification_approaches..jpg ‎(563 × 600 pixels, file size: 93 KB, MIME type: image/jpeg)

Captions

Captions

Add a one-line explanation of what this file represents
This biology image could be re-created using vector graphics as an SVG file. This has several advantages; see Commons:Media for cleanup for more information. If an SVG form of this image is available, please upload it and afterwards replace this template with {{vector version available|new image name}}.


It is recommended to name the SVG file “Schematic depiction of two affinity purification approaches..svg”—then the template Vector version available (or Vva) does not need the new image name parameter.
This biology image was uploaded in the JPEG format even though it consists of non-photographic data. This information could be stored more efficiently or accurately in the PNG or SVG format. If possible, please upload a PNG or SVG version of this image without compression artifacts, derived from a non-JPEG source (or with existing artifacts removed). After doing so, please tag the JPEG version with {{Superseded|NewImage.ext}} and remove this tag. This tag should not be applied to photographs or scans. If this image is a diagram or other image suitable for vectorisation, please tag this image with {{Convert to SVG}} instead of {{BadJPEG}}. If not suitable for vectorisation, use {{Convert to PNG}}. For more information, see {{BadJPEG}}.
Description
English: A. Tandem affinity chromatography: a protein of interest is first engineered to contain a Protein A (filled in circle) tag, a Tobacco Echo Virus recognition site (TEV, filled in triangle), and a calmodulin binding peptide (CBP) tag (filled in ellipse). Extracts are made from cells expressing the tagged protein, which should contain associated proteins and contaminants. These complexes are bound to an IgG column, and washed to remove majority of contaminants. TEV protease is then used to elute the semi-purified protein complexes which are subsequently absorbed onto a calmodulin column. After further washing, purified protein complexes containing the protein of interest and its associated proteins are eluted by calcium chelation (EGTA) and identified using LC-MS/MS.
Date Published July 14, 2008.
Source

[1] Direct
StemBook Figure 3A Schematic depiction of two affinity purification approaches.

  • Wang, J., Trowbridge, J.J., Rao, S. and Orkin, S.H., Proteomic studies of stem cells (July 14, 2008), StemBook, ed. The Stem Cell Research Community, StemBook, doi/10.3824/stembook.1.4.1, http://www.stembook.org.
Author Wang, J., Trowbridge, J.J., Rao, S. and Orkin, S.H., Proteomic studies of stem cells (July 14, 2008), StemBook, ed. The Stem Cell Research Community, StemBook, doi/10.3824/stembook.1.4.1, http://www.stembook.org.
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 3.0 Unported license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current19:04, 5 April 2013Thumbnail for version as of 19:04, 5 April 2013563 × 600 (93 KB)Smallbot (talk | contribs)Uploading CC-BY images from the the StemBook http://www.stembook.org/ 151/173

You cannot overwrite this file.

There are no pages that use this file.

File usage on other wikis

The following other wikis use this file:

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Schematic_depiction_of_two_affinity_purification_approaches..jpg&oldid=495030519"