File:PiRNA pathway mutants show germline defects in Drosophila..jpg

English: A.

armi and aub mutant males show overexpression of Stellate protein in the testes. Testes were stained for DNA (red) and Stellate (green). Scale bar is 20 μM. Reprinted from Klattenhoff et al. (2007), with permission from Elsevier. B. DNA damage accumulation in the female germline of aub mutant germaria. Ovaries are labeled for γ-H2Av, a marker of DNA double-strand breaks. Scale bar is 20 μM. Reprinted from Klattenhoff et al. (2007), with permission from Elsevier. C.

piwi is required for germline stem sell division during oogenesis. DAPi stained images of 0–1 day old, wt and piwi1 mutant ovaries showing loss of most of the developing egg chambers in piwi1, and germaria lacking germ lines (Ge). S1 refers to the first stage egg chamber budding off the germarium in the wt. Scale bar is 50 μM. Reprinted from Cox et al. (1998), with permission from Cold Spring Harbor Press Laboratory.