File:Molecular-and-Morphological-Evidence-Reveals-a-New-Species-in-the-Phyllomedusa-hypochondrialis-pone.0105608.g003.jpg

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English: Karyotype of Phyllomedusa rustica sp. n. based on Giemsa staining (A), C-banding (B), DAPI staining after C-banding (C), and chromosomes submitted to Mytramicim (MM) staining after C-banding, silver impregnation via the Ag-NOR method (D) and FISH experiments with a nucleolar 28S rDNA probe. Arrows in (A) indicate secondary constrictions. The inset in (C) shows the homomorphic condition of homologue pair 9. In D, the chromosome pairs submitted to MM staining exhibit brilliant fluorescence, coinciding with the secondary constrictions detected by Giemsa-staining. The arrowheads indicate NOR sites in (E) and (F).
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Source Image file from Bruschi D, Lucas E, Garcia P, Recco-Pimentel S (2014). "Molecular and Morphological Evidence Reveals a New Species in the Phyllomedusa hypochondrialis Group (Hylidae, Phyllomedusinae) from the Atlantic Forest of the Highlands of Southern Brazil". PLOS ONE. DOI:10.1371/journal.pone.0105608. PMID 25141279. PMC: 4139387.
Author Bruschi D, Lucas E, Garcia P, Recco-Pimentel S
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