File:Lateral flow immunochromatographic test KZ.jpg

A colloidal gold-protein G conjugate was applied (11 µL per centimeter of the strip) to a conjugate release matrix, using a dispenser (XYZ3060 Dispense Platform, BIODOT,, USA), at a dilution corresponding to OD520 = 1.0. K: The test zone was formed on a polyester-backed nitrocellulose membrane using B. abortus rOMP28 (Recombinant outer membrane protein of Brucella spp); 2 µL of rOMP28 (1.0 mg/mL in distilled water) was applied per centimeter of the strip. Z: The control zone was formed on a polyester-backed nitrocellulose membrane (MDA, India) using rabbit anti-bovine IgG antibodies; 2 µL of the antibodies (5 mg/mL in 50 mM potassium phosphate buffer, pH 7.4, containing 0.1 M NaCl) was applied per centimeter of the strip. KZ is abreviation of Kazakhstan.

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