File:HBsAg ELISA final reaction ready for reading.jpg
Original file (4,160 × 2,340 pixels, file size: 1.03 MB, MIME type: image/jpeg)
Captions
Captions
Summary[edit]
| Description |
English: The HBsAg ELISA (Enzyme-Linked Immunosorbent Assay) final reaction ready for reading refers to the endpoint of the test where the results are interpreted. This test is used to detect the presence of the Hepatitis B surface antigen (HBsAg) in a blood sample. The presence of HBsAg indicates an active Hepatitis B infection. Here's what the process involves:
Sample Preparation: A blood sample is collected from the patient. The blood is processed to isolate the serum, which contains antibodies and antigens. ELISA Procedure: The ELISA procedure involves several steps: Coating: A well of a microtiter plate is coated with specific antibodies that will bind to the HBsAg. Incubation: The serum sample is added to the well and allowed to incubate. If HBsAg is present in the sample, it will bind to the antibodies immobilized on the well. Washing: The well is washed to remove any unbound components. Detection: Enzyme-conjugated antibodies that specifically bind to HBsAg are added. If HBsAg is present, these enzyme-conjugated antibodies will bind to it. Substrate Addition: A substrate that reacts with the enzyme is added. The reaction produces a color change that indicates the presence of HBsAg. Endpoint Reading: After the substrate has been added, a color change occurs in the presence of HBsAg. The intensity of the color change is proportional to the amount of HBsAg in the sample. The microtiter plate is then placed in a spectrophotometer to measure the optical density (OD) of the color. Interpretation: The OD readings are used to determine the presence or absence of HBsAg. A higher OD reading indicates a stronger reaction and a higher concentration of HBsAg in the sample. Results interpretation: Positive: A high OD reading suggests the presence of HBsAg, indicating an active Hepatitis B infection. Negative: A low OD reading suggests the absence of HBsAg, indicating that Hepatitis B infection is not currently present. Borderline: In some cases, the OD reading might fall in a borderline range, requiring further testing or follow-up. |
| Date | |
| Source | Own work |
| Author | Ajay Kumar Chaurasiya |
Licensing[edit]
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:46, 26 August 2023 | | 4,160 × 2,340 (1.03 MB) | Ajay Kumar Chaurasiya (talk | contribs) | Uploaded own work with UploadWizard |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
