File:Fpls-02-00042-g004.png

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English: Detection of fucose-containing N-glycans in protein extracts from transgenic XylT_RNAi and wild type tomato fruits. (A) Immunoblot using anti-xylose IgG antibodies; (B) Immunoblots using anti-fucose IgG antibodies; (C) Silver staining of SDS-PAGE. Protein extracts from green (lanes 1–3), orange (lanes 4–6), and red (lanes 7–9) tomato fruits were separated by SDS-PAGE and probed for xylose- or fucose-containing epitopes. Lanes 1, 4, and 7, protein extracts from wild type controls; lanes 2, 5, and 8, protein extracts from the T2 generation of line XylT_RNAi 3–16–10; lanes 3, 6, and 9, protein extracts from the T2 generation of line XylT_RNAi 12–15–1. Arrow heads indicate protein standards of 95, 55, and 36 kDa, respectively.
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Source doi:10.3389/fpls.2011.00042
Author Kathrin Elisabeth Paulus, Vera Mahler, Martin Pabst, Karl-Heinz Kogel, Friedrich Altmann, Uwe Sonnewald

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