File:Fpls-02-00020-g002.png

English: Subcellular localization of Arabidopsis Tom1 proteins, GFP-Syp21, YFP-Rha1, and RFP-Ara7. BY-2 cells were either transiently (co-)transformed with either (A) various Myc-tagged Tom1 proteins and GFP-Syp21, (B) the same Myc-Tom1 proteins on their own, (C) Tom1A-Myc and GFP-Syp21, (D) GFP-Tom1A and RFP-Ara7, (E) GFP-Syp21 and RFP-Ara7 or YFP-Rha1 and RFP-Ara7, or (F) GFP-Syp21 and RFP-Ara7. Cells were then either formaldehyde-fixed and processed for immuno-epifluorescence microscopy or [in (F)] CLSM. Alternatively, cells [in (D) bottom row) were viewed living (i.e., non-fixed) using epifluorescence microscopy. Each micrograph is labeled at the top left with the name of the (co-)expressed protein construct. Also shown is the corresponding differential interference contrast (DIC) image or [in (F)] merged image for each set of (co-)transformed cell(s). Hatched boxes in [(A) and (C–F)] represent the portion of cells shown at higher magnification and colorized in the panels below. The yellow color in the merged images indicates co-localization between co-expressed proteins; white arrowheads [in (E,F)] also indicate obvious protein co-localization, while the open arrowheads in (E,F) indicate obvious non-co-localization. Bar = 10 μm.