File:Fert7.JPG
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Summary[edit]
| Description | bovine fertilization |
| Date | 9/11/08 |
| Source | Own work |
| Author | Mtengows |
Immunocytochemistry of ejaculated bull sperm incorporation into zona pellucida- intact bovine oocytes. The images A–D were 8 h post-sperm addition and images E–H were 12 h post-sperm addition. Actin localization (A and E) as indicated by Texas Red–phalloidin conjugate suggests that actin localization around the sperm head may be transient, whereas the oolemma microvilli have a consistent localization pattern throughout. Sperm heads can be localized with DAPI staining (B and F) as well as the extrusion of the polar body (B). A microtubule sperm aster can be immunolocalized in G, but not in C. Nomarski microscopy confirms the presence of a single sperm bound to the surface of the oocyte; incorporating sperm is indicated with an arrow. Images originally published in: Tengowski, MW (2004) Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development. Methods in Molecular Biology (Clifton, N.J.), 253, 165-99.
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Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 22:25, 11 September 2008 | | 602 × 864 (55 KB) | Mtengows (talk | contribs) | {{Information |Description=bovine fertilization |Source=self-made |Date=9/11/08 |Author= Mtengows |Permission= |other_versions= }} Immunocytochemistry of ejaculated bull sperm incorporation into zona pellucida- intact bovine oocytes. Th |
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