File:Fert6.JPG
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Summary[edit]
| Description | bovine fertilization |
| Date | 9/11/08 |
| Source | Own work |
| Author | Mtengows |
Immunocytochemistry of ejaculated bull sperm incorporation into zona pellucida-free bovine oocytes. Images A–D (120 min) and E–H (240 min) display different stages of incorporation post-sperm addition. Actin localization (A and E) as indicated by Texas Red–phalloidin conjugate shows a localization at the sperm head, oolemma, and site of polar body extrusion (A). Multiple sperm heads can be seen bound to the surface of the oocytes as detected by DAPI staining (B and F). At this time point of sperm incorporation, there was no significant microtubule immunolocalization (meiotic spindle was visible in C). Nomarski microscopy confirms the presence of numerous sperm bound to the surface of the oocytes; incorporating sperm are indicated with arrows. Images originally published in: Tengowski, MW (2004) Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development. Methods in Molecular Biology (Clifton, N.J.), 253, 165-99.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 22:24, 11 September 2008 | | 577 × 823 (52 KB) | Mtengows (talk | contribs) | {{Information |Description=bovine fertilization |Source=self-made |Date=9/11/08 |Author= Mtengows |Permission= |other_versions= }} Immunocytochemistry of ejaculated bull sperm incorporation into zona pellucida-free bovine oocytes. Image |
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