File:EMT and vimentin in confined spaces.jpg

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Description Figure 5. EMT and vimentin in confined spaces. a Micropillar arrays for confined migration fabricated using an elastomeric silicone with 10 μm spacing and height. b Mammary epithelial cells (MCF-10A) migrate as individuals breaking away from a collective front after EMT induction via the Snail transcription factor. Immunofluorescence staining of the nucleus (blue), E-cadherin (green), and vimentin (red). c Transwell (Boyden Chamber) assay for migration through confined pores. d Nuclear morphology of wild type and vimentin knockout fibroblasts after traversing membrane. Immunofluorescnece staining of the nucleus (blue), Lamin-A (green), and Lamin B (red).
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Source https://biosignaling.biomedcentral.com/articles/10.1186/s12964-021-00713-2#rightslink The epithelial-mesenchymal transition and the cytoskeleton in bioengineered systems. Cell Commun Signal 19, 32 (2021). https://doi.org/10.1186/s12964-021-00713-2
Author Leggett, S.E., Hruska, A.M., Guo, M. et al.
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current20:57, 16 May 2024Thumbnail for version as of 20:57, 16 May 20241,614 × 1,384 (1.12 MB)Rasbak (talk | contribs){{Information |description=Figure 5. EMT and vimentin in confined spaces. a Micropillar arrays for confined migration fabricated using an elastomeric silicone with 10 μm spacing and height. b Mammary epithelial cells (MCF-10A) migrate as individuals breaking away from a collective front after EMT induction via the Snail transcription factor. Immunofluorescence staining of the nucleus (blue), E-cadherin (green), and vimentin (red). c Transwell (Boyden Chamber) assay for migration through confi...

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