File:CPU time spent by each program when aligning increasing sequence lengths.png

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English: MAFFT FFT-NS-2 was the fastest program for aligning all tested sequence sizes. T-Coffee was the slowest program, taking longer than Clustal W, MAFFT (FFT-NS-2 and L-INS-i), Kalign, and POA for the smallest sequence set (100 amino acids), and having the second worst average time for most sequence sizes.
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Source https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-7-471
Author Paulo AS Nuin,Zhouzhi Wang & Elisabeth RM Tillier

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