File:Boletus roodyi B. Ortiz, D.P. Lewis & Both 636044.jpg
Original file (3,087 × 2,542 pixels, file size: 3.74 MB, MIME type: image/jpeg)
Captions
Captions
Summary[edit]
| Description |
Boletus roodyi B. Ortiz, D.P. Lewis & Both Image location: Washington Crossing State Park, Mercer Co., New Jersey, USA > Two fruiting bodies growing next to each other in mixed deciduous woods; the surrounding trees are maples and a single hickory, with oaks ~15-20 yards away. I’ve been visiting this spot for over a decade, but have never seen these boletes there before. > The colors are presented accurately in the pix. Interestingly, the older fb had a richer, brighter pileipellis color than the younger one. The pore surface is dingy yellow to greenish-yellow in the older fb over time. The pore surface of the older fruitbody appeared to have bruised very faintly grayish-blue when fresh, resolving faint brownish. The stipe is light lemon-yellow, with some burgundy at the base and where the slug damage occurred in the large fb. Other than that, there is absolute no hint of red in the stipe. There is no bluing on the stipe surface upon handling or injury. The exposed context is whitish to pale yellow in the cap context and light yellow in the stipe, appears to be unchanging in the first 10-15 min. However, a narrow band of very faint and diffuse bluing was observed directly above the tubes only, resolving to faint grayish and then back to the original context color. Large fb: Cap = 12-13.5 cm wide and up to 2 cm thick; tube layer = up to 1 cm thick; stipe = 6.5 cm long and uniformly 2.5 cm wide. Small fb: cap = 3.8 cm wide and up to 1 cm thick; tube layer = 2.5 mm thick; stipe = 6.2 cm long and uniformly 1.2-1.4 cm wide. Chemical Tests (performed on the small fb, unless stated otherwise): KOH = mustard yellow on pileipellis; pale orangish on context; brownish on tubes (larger fb only). NH4OH = mustard yellow on piliepillis (a bit paler than KOH); no reaction on context. FeSO4 = medium brown, resolving bluish on pileipellis; pale slate on context. DNA Sequencing Results & Discussion: > A clean and contiguous nrLSU sequence of 1441 bps was obtained from this material. GenBank BLAST searches of the full-length and partial (first 978 characters) sequences didn’t return any meaningful results. Sequence similarity didn’t exceed 97% even for the top-scoring hits, meaning that other loci would be required for establishing its generic position in the Boletaceae. Nothing made sense in terms of morphological semblance between this bolete and GenBank hits either. Alignment of this sequence with that of my own Boletus miniato-olivaceus voucher, obs 168189, revealed that the 1382 bps overlay had 37 mismatching base-pairs, corresponding to only 97.3% similarity. > A clean and contiguous nrITS sequence of 721 bps was obtained for this material. A GenBank BLAST search yielded no close hits. The highest observed identity was only ~89%. Based on chemical features: ITS & LSU sequences match those of the type material (see comment below)
|
||
| Date | |||
| Source |
|
||
| Author |
|
Licensing[edit]
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
|
This file, which was originally posted to
|
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 04:03, 12 August 2018 | | 3,087 × 2,542 (3.74 MB) | Leoboudv (talk | contribs) | =={{int:filedesc}}== {{Information |Description='''''Boletus roodyi''''' B. Ortiz, D.P. Lewis & Both Image location: Washington Crossing State Park, Mercer Co., New Jersey, USA '''>''' Two fruiting bodies growing next to each other in mixed deciduous woods; the surrounding trees are maples and a single hickory, with oaks ~15-20 yards away. I’ve been visiting this spot for over a decade, but have never seen these boletes there before. '''>''' The colors are presented accuratel... |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
