File:Aves EMT in the formation of the primitive streak.jpg

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Fig 2. EMT in the formation of the primitive streak (PS).

(A–E) Images from a time-lapse sequence of entire embryos (Video 1), showing the uniform epiblast 6 hr (A, stage EG&K XII) and just before primitive streak formation (B, stage EG&K XIV), the first appearance of the primitive streak (C, stage HH2), accumulation of mesoderm beneath the flat streak (D, stage HH3), appearance of a groove in the PS and emigration of mesoderm (E, stage HH3+). (F–K) SEM of fractured embryos before (F–H) and after (I–K) streak formation. White arrows indicate possible EMT before PS formation. (L–P) SEM of fractured PS, showing EMT cells with various degrees of apical constriction and basolateral expansion (classified as ‘ingression stages 1–5’). (Q) This embryo was cultured for 1 hr after electroporation of a control, fluorescent morpholino into the entire epiblast at stage XI, then sectioned sagitally and viewed under fluorescence. Labelled cells in the epiblast show similar morphologies to those in SEMs (panels L–P, ‘ingression stages 1–5’). (R) This embryo was cultured for 4 hr after electroporation of a control, fluorescent morpholino into the entire epiblast at stage XI, then fixed (at stage XII), sectioned sagitally and stained with anti-fluorescein antibody (brown). The section shows several cells that have left the epiblast and are now in the underlying space throughout the anterior-posterior extent of the embryo (arrows).
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Source https://doi.org/10.7554/eLife.01817 Local cell interactions and self-amplifying individual cell ingression drive amniote gastrulation eLife 3:e01817.
Author Octavian Voiculescu Lawrence Bodenstein I-Jun Lau Claudio D Stern
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© 2014, Voiculescu et al.

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current20:37, 16 April 2024Thumbnail for version as of 20:37, 16 April 2024856 × 1,500 (351 KB)Rasbak (talk | contribs){{Information |description=EMT in the formation of the primitive streak (PS).<br> (A–E) Images from a time-lapse sequence of entire embryos (Video 1), showing the uniform epiblast 6 hr (A, stage EG&K XII) and just before primitive streak formation (B, stage EG&K XIV), the first appearance of the primitive streak (C, stage HH2), accumulation of mesoderm beneath the flat streak (D, stage HH3), appearance of a groove in the PS and emigration of mesoderm (E, stage HH3+). (F–K) SEM of fractured em...

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