File:41598 2016 Article BFsrep18696 Fig2 HTML.webp
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English: Morphogenesis of Singapore Grouper Iridovirus (SGIV) capsids in the viral assembly site (VAS).
(A–F) Main panel, electron micrograph depicting different viral intermediates during capsid assembly; Top right panel, insets of the central slice through a tomogram; Bottom right panel, isosurface rendition of the corresponding viral intermediates (yellow). (A,B) Membranous vesicles, tubules and sheets, scale bar = 100 nm. (C) Crescent-shaped structures with two shells; scale bar = 50 nm. (D–F) Electron dense materials accumulate on the inner layer as the capsid is being filled. As the packing process continues, the two shell crescent-shaped structure curves to form icosahedral capsids with an opening (black arrow). Mature virions (white arrow) with a dense core are eventually formed. No opening was observed on the fully formed mature particles; scale bars = 100 nm. (G) The distribution of capsid intermediates observed in infected GECs maintained at 4 °C and 27 °C, at 1 or 2 hpi (hours post infection). |
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Fig 2 at https://www.nature.com/articles/srep18696 Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells. In: Sci Rep 6, 18696 (2016). doi:10.1038/srep18696  |
| Author | Yang Liu, Bich Ngoc Tran, Fan Wang, Puey Ounjai, Jinlu Wu, Choy L. Hew |
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