File:Ацетокарминовый метод.jpg
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Captions
Captions
Pollen grains of common pine (Pinus silvestris) stained with acetocarmine
Summary[edit]
| Description |
Русский: Определение жизнеспособности пыльцы Сосны обыкновенной (Pinus silvestris) с помощью ацетокарминового метода. Для этого собирают пыльцу и либо сразу окрашивают, либо фиксируют в фиксаторе Карнуа два часа или больше, потом переносят пыльцевые зёрна в 80%-этиловый спирт, где и хранят их. Окраску ацетокармином проводят в любое время.
Пыльцевые зёрна помещают на предметное стекло, капают пару капель ацетокармина, накрывают покровным стеклом и осторожно нагревают над пламенем спиртовки. Ацетокармин - ядерный краситель, поэтому у фертильных пыльцевых зёрен сосны зернистая цитоплазма, вегетативная клетка, антеридиальная клетка и др. окрашиваются в карминово-красный цвет. Стерильные пыльцевые зёрна кармином не окрашиваются или окрашиваются неравномерно.
English: Determination of pollen viability of Pinus silvestris using the acetocarmine method. For this purpose, pollen is collected and either stained immediately or fixed in Carnua fixative for two hours or more, then pollen grains are transferred into 80% ethyl alcohol, where they are stored. Acetocarmine staining is carried out at any time.
Pollen grains are placed on a slide, a couple of drops of acetocarmine are dropped, covered with a coverslip, and gently heated over a spirit flame. Acetocarmine is a nuclear dye; therefore, in fertile pine pollen grains the granular cytoplasm, the vegetative cell, the antheridial cell, etc. are stained carmine red. Sterile pollen grains are not stained with carmine or are stained irregularly. Translated with www.DeepL.com/Translator (free version)English: Pollen grains of common pine (Pinus silvestris) stained with acetocarmine |
| Date | |
| Source | Own work |
| Author | Anatoly Mikhaltsov |
Licensing[edit]
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This image was uploaded as part of Russian Science Photo Competition 2023.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 09:27, 29 May 2023 | | 3,543 × 2,487 (1.73 MB) | Anatoly Mikhaltsov (talk | contribs) | Uploaded own work with UploadWizard |
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