File:Two-color MINFLUX image of mitochondria.jpg
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Summary
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English: The mitochondrial protein TOM20 (green) and mitochondrial DNA (red) were labeled in mammalian cells with indirect immunofluorescence using secondary antibodies coupled to sCy5 and CF680. Two-color confocal (A) and MINFLUX (B) imaging was performed using a ratiometric detection strategy. The labeling density of the two structures differs. For TOM20, single proteins are labeled in the mitochondrial membrane, whereas numerous binding sites are labeled in the mitochondrial DNA. MINFLUX enables visualizing and discriminating between the two structures. Scale bars: 500 nm. |
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| Date | |||
| Source | Images were provided by abberior Instruments GmbH | ||
| Author | abberior Instruments GmbH, contact person: Dr. Frederik Köpper | ||
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 11:01, 11 August 2023 | | 827 × 632 (98 KB) | AByolia (talk | contribs) | Uploaded a work by abberior Instruments GmbH, contact person: Dr. Frederik Köpper from {{subst:PP}} Images were provided by abberior Instruments GmbH with UploadWizard |
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