File:Trüpsiini kristallid polariseeritud valguses.jpg

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English: Crystals of enzyme trypsin under Nikon SMZ800 microscope using polarizing lens. Polarizer is a simple solution to distinguish protein crystals from salt crsytals due to their optical differences, saving time and money not measuring salt crystals in diffractometer. Crystals were grown in the presence of benzamidine at room temperature and using seeding technique for obtaining crystals of better quality.
Eesti: Trüpsiini kristallid Nikon SMZ800 mikroskoobi all läbi polariseeriva filtri. Polariseeriv filter annab kristallimise teaduses lihtsa võimaluse eristada valgu kristalli soolakristallist, kuna valgu kristall on valguse suhtes enamikel juhtudel anisotroopne, soolakristallid tihti mitte. Soolakristalli mõõtmine difraktomeetri või sünkrotroniga oleks aga aja ja ressursside kulu. Trüpsiin on oluline ensüüm seedeelundite töös, lagundades valke väiksemateks ühikuteks. Kristallid kasvatati 22 kraadi Celsiuse juures bensamidiini (trüpsiini inhibiitor, soodustades kristallide ning korrektsema kristallstruktuuri teket) juuresolekul ning piisava suurusega kristallide saamiseks kasutati nn külvamistehnikat (seeding). Kristallimisel varieeriti pH ning soola (ammooniumsulfaat) kontsentratsiooni, pildil on parima kvaliteediga kristallid kõigi katsetatud tingimuste hulgast ning sobilikud kristallstruktuuri mõõtmiseks difraktomeetris. Pilt on tehtud nutitelefoniga Samsung Galaxy SII(kaamerasilma suurus on sobilik mikroskoobi okulaari avaga) Tromsø Ülikooli Norstruct laboris.
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Author Taavi Ivan

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current17:22, 18 October 2012Thumbnail for version as of 17:22, 18 October 20121,865 × 2,055 (1.28 MB)Taavi.Ivan (talk | contribs){{Information |Description ={{en|1=Crystals of enzyme trypsin under Nikon SMZ800 microscope using polarizing lens. Polarizer is a simple solution to distinguish protein crystals from salt crsytals due to their optical differences, saving time and mo...

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