File:The mesovarium and cranial suspensory ligament tether the ovary to the rest of the urogenital complex.jpg
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Figure 5—figure supplement 1.The mesovarium and cranial suspensory ligament tether the ovary to the rest of the urogenital complex. (A) Confocal images of whole ovary/mesonephros complexes at E15.5, E16.5, and E18.5 immunostained for aSMA (green), and counterstained with Hoechst nuclear dye (grayscale). Samples in the top two rows were imaged from the dorsal side and samples in the bottom row from the medial side. (B) 3D models generated by isosurface segmentation of lightsheet images taken of whole ovaries at E14.5, E17.5 and P0 immunostained for FOXL2 (cyan) and TNC (E14.5, red) or labeled with AF647-Hydrazine to reveal Elastin expression (E17.5, P0, red). All samples were counterstained with Hoechst nuclear dye (grayscale). Top panels represent the dorsal view, middle panels represent the medial view, and small bottom panels illustrate ventral and lateral views of the same ovary. White dashed arrows show the direction to the body wall. Yellow asterisks indicate the location of the infundibulum of the presumptive oviduct for reference. Compasses on the bottom left of each panel indicate the orientation of the ovary for the entire row: CSL, cranial suspensory ligament; D, dorsal; H, hilum; L, lateral; M, medial; MD, Müllerian duct; Mm, mesometrium; Mn, mesonephros; Ms, mesosalpinx; Mv, mesovarium; OA, ovarian artery; OC, ovarian capsule; Ov, ovary; Ovi, oviduct; Ut, uterus; V, ventral. Scale bars, 100 μm. |
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| Source | https://elifesciences.org/articles/81088 Jennifer McKey, Dilara N. Anbarci, Corey Bunce, Alejandra E. Ontiveros, Richard R. Behringer, Blanche Capel Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii, bioRxiv 2021.05.21.445181; doi: https://doi.org/10.1101/2021.05.21.445181 |
| Author | Jennifer McKey, Dilara N. Anbarci, Corey Bunce, Alejandra E. Ontiveros, Richard R. Behringer, Blanche Capel |
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© 2022, McKey et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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| current | 10:22, 6 August 2024 | | 3,516 × 3,093 (1.46 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 5—figure supplement 1.The mesovarium and cranial suspensory ligament tether the ovary to the rest of the urogenital complex. (A) Confocal images of whole ovary/mesonephros complexes at E15.5, E16.5, and E18.5 immunostained for aSMA (green), and counterstained with Hoechst nuclear dye (grayscale). Samples in the top two rows were imaged from the dorsal side and samples in the bottom row from the medial side. (B) 3D models generated by isosurface segmentation... |
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