File:The Journal of laboratory and clinical medicine (1915) (14578377298).jpg

Identifier: journaloflaborat05cent (find matches)
Title: The Journal of laboratory and clinical medicine
Year: 1915 (1910s)
Authors: Central Society for Clinical Research (U.S.)
Subjects: Biological Assay Diagnosis, Laboratory Disease Medicine Medicine Research
Publisher: St. Louis, C. V. Mosby
Contributing Library: Gerstein - University of Toronto
Digitizing Sponsor: University of Toronto

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hs, and have given excellent service. (Fig. 2.) Agalvanized iron water-bath made large enough to accommodate 20 regulationWassermann test tube racks (200 sera specimens) was used for incubation.(Fig. 3.) The cost of the inactivating and incubating baths was very low com-pared to that of commercial water-baths. 100 THE JOURNAL OF LABORATORY AXD CLIXICAL MEDICINE An amicomplemcnlary control, besides known negative and positive controls,was run fur each scrum. The results were read immediately and two hoursafter the final incubation. The results of the latter reading were those reported. EXPRESSION or RESULTS According to the custom of the Medical Department, U. S. Armv, the resultsof the Wassermann tests are expressed as follows:-;—;- Diagnostic of syphilis. + Not diagnostic unless there is a clear history of symptoms.± Not a diagnostic reaction, but of value in indicating result of treatment.— A single negative does not exclude syphilis, particularly in the primaryand late stages.
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In my opinion this nomenclature does not indicate the results .so clearlyas the four-)ilus reactions used in the majority of laboratories. Compared tothe four-plus system of recording the army double-plus reaction correspondswith the four-plus; the single-plus with the three- and two-plus; and the plus-minus with the one-plus reaction. Uniformity of expression of results is to begreatly desired, particularly those results indicating the course of treatment. Smion advocates the use of percentage in designating the amount of com-plement fixation taking place. I should therefore ))roi)ose to designate as aunit such quantity of the sy()hililic antibody as re(iuires sixty minutes incubationat from 37° to 40° C. to bring about .complete fixation of a standard amount ofcomplement. Such amount of the syphilitic antibody as is capable for fixingthe same quantity of complement without any incubation whatsoever I shoulddesignate as sixty units and a reaction of the latter type 1 should term a

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