File:The Biological bulletin (19756701664).jpg

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Title: The Biological bulletin
Identifier: biologicalbullet191mari (find matches)
Year: [1] (s)
Authors: Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983
Subjects: Biology; Zoology; Biology; Marine Biology
Publisher: Woods Hole, Mass. : Marine Biological Laboratory
Contributing Library: MBLWHOI Library
Digitizing Sponsor: MBLWHOI Library

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About This Book: Catalog Entry
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266 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS
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Figure 1. An example of an ionophore-inducedCd** wave crossing a zebrafish egg. Panel A illustrates a transmitted light image of an unactivated egg. funds B through H are successive (microchannel-plate-generated) fluorescent images showing a Ca~+ wave crossing the egg at a velocity of about 10 iim/s (ionomycin solution added at Panel B, inj. indicates the injection site). Panel I is a second transmitted light image collected after the passage of the wave, clearly illustrating that the egg had been activated (arrowhead indicates the rising chorion). Panels J through L show that activated (but unfertilized) eggs loaded with Fluo-3 undergo normal ooplasmic segregation, forming a regular blastodisc (L is an overlay ofJ and K). It i.v clear that indicator dye injected into the center of an unactivated egg is carried via f>lasmic streaming (indicated by arrow in Panel J) into the lormmg hlastodtsc. PanelJ (60 min post-activation) illustrates a fidly elevated chorion. Scale bars in Panels A and. 1 equal 100 tun

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7 August 2015


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