File:The-cation-channel-TRPA1-tunes-mosquito-thermotaxis-to-host-temperatures-elife-11750-fig3.jpg

English: (A) Representative bright field (left) and fluorescence (right) images of wild-type and AaegTRPA1-/- female pupae marked by ubiquitous expression of enhanced cyan fluorescent protein (ECFP). Scale bars: 0.5 mm. (B) Schematic of capillary feeding (CAFE) assay. (C,D) Sucrose preference over sucrose containing the indicated concentration of N-methylmaleimide (C, n = 10–12 trials per condition) or denatonium benzoate (D, n = 7 trials per condition) for mosquitoes of the indicated genotypes (NS, not significant; *p < 0.05, **p < 0.01; one-way ANOVA with Bonferroni correction compared to wild-type). (E) Percent of mosquitoes of indicated genotypes on Peltier during seconds 90–180 of stimuli of indicated temperature (mean ± s.e.m., n = 6–9 trials per genotype; ***p < 0.001; repeated measures one-way ANOVA with Bonferroni correction). (F) Heat maps showing mean mosquito occupancy for the indicated genotypes on the Peltier (red square) and surrounding area, during seconds 90–180 of each stimulus period. Bold borders indicate stimuli with responses significantly different from wild-type in (E) (p < 0.05; repeated-measures ANOVA with Bonferroni correction). (G) Mean percent of mosquitoes of indicated genotypes on Peltier during seconds 0–180 of thermal stimuli 40–60°C and during subsequent re-presentation of 40°C. Timespans with statistically significant increases in AaegTRPA1-/- mutant Peltier occupancy compared to wild-type are indicated by purple lines (calculated from 15 second bins; p < 0.05; one-way ANOVA with Bonferroni correction).